Luke Letlow graduated from Ouachita Christian High School and earned a Bachelor of Science in computer information systems from Louisiana Tech University. He held leadership roles in student politics, serving as Chairman of the Louisiana Tech College Republicans and the Louisiana Federation of College Republicans. Letlow began his career in politics as a district and intergovernmental affairs director for Bobby Jindal, transitioning to the private sector as a director for QEP Resources. He later managed and served as Chief of Staff for Ralph Abraham.

Luke Letlow's political identity is marked by a curious tension between his advocacy for reopening the economy during the COVID-19 pandemic and his sporadic mask-wearing, which reveals a calculated approach to balancing public health concerns with economic interests. His background in conservative leadership, including serving as Bobby Jindal's district director and chief of staff to Ralph Abraham, underscores a deeply rooted ideological foundation. Letlow's stance on COVID-19 restrictions, juxtaposed with his personal health outcomes, invites scrutiny into how he reconciled his policy recommendations with personal risk.