Anne-Marie Mastraccio began her career as a dental hygienist in Boston before relocating to Maine. She has spent over three decades in public service, most recently serving as mayor of Sanford. Throughout her tenure in the Maine House of Representatives, she has focused on economic development and labor policies, including co-sponsoring a bill to address tipped minimum wage adjustments. Her experience spans across various legislative committees, notably those concerning health coverage, insurance, financial services, and government oversight. Additionally, she has been actively involved in her husband's optometric practice for over 20 years.

Anne-Marie Mastraccio's political identity is marked by a calculated approach to economic policies, particularly in balancing regional industrial interests with labor rights. Her legislative actions, such as co-sponsoring a bill to reverse tipped minimum wage increases, reveal a nuanced stance on labor regulations. Despite her commitment to economic development and skills training, Mastraccio's policy decisions suggest a strategic alignment with business interests over worker protections, raising questions about her broader economic philosophy.